research article
AMPA receptor imaging by infrared scanning near-field optical microscopy
Infrared scanning near-field optical microscopy is used to detect AMPA-type glutamate receptor clusters in hippocampal neuron cells. AMPA's GluR2-subunits were marked with the fluorophore Alexa488 and high-resolution micrographs were taken at 6.25 µm wavelength, corresponding to the maker's strong infrared absorption. The use of infrared light avoids photobleaching and makes this approach suitable for long-term observations; this technique can be used in general to localize different IR-absorbing molecules over the complex background of other cell components.
Type
research article
Web of Science ID
WOS:000257704500021
Author(s)
Generosi, Johanna
Kropf, Michel
Hirling, Harald
Catsicas, Stefan
Tolk, Norman H.
Piston, David W.
Cricenti Phys., Antonio
Date Issued
2008
Publisher
Published in
Volume
5
Issue
8
Start page
2641
End page
2644
Editorial or Peer reviewed
REVIEWED
Written at
EPFL
EPFL units
Available on Infoscience
June 10, 2008
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