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research article

AMPA receptor imaging by infrared scanning near-field optical microscopy

Generosi, Johanna
•
Kropf, Michel
•
Margaritondo, Giorgio  
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2008
Physica Status Solidi C

Infrared scanning near-field optical microscopy is used to detect AMPA-type glutamate receptor clusters in hippocampal neuron cells. AMPA's GluR2-subunits were marked with the fluorophore Alexa488 and high-resolution micrographs were taken at 6.25 µm wavelength, corresponding to the maker's strong infrared absorption. The use of infrared light avoids photobleaching and makes this approach suitable for long-term observations; this technique can be used in general to localize different IR-absorbing molecules over the complex background of other cell components.

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Type
research article
DOI
10.1002/pssc.200779116
Web of Science ID

WOS:000257704500021

Author(s)
Generosi, Johanna
Kropf, Michel
Margaritondo, Giorgio  
Hirling, Harald
Catsicas, Stefan
Johnsson, Kai  
Tolk, Norman H.
Piston, David W.
Cricenti Phys., Antonio
Date Issued

2008

Publisher

Wiley

Published in
Physica Status Solidi C
Volume

5

Issue

8

Start page

2641

End page

2644

Editorial or Peer reviewed

REVIEWED

Written at

EPFL

EPFL units
LIP  
Available on Infoscience
June 10, 2008
Use this identifier to reference this record
https://infoscience.epfl.ch/handle/20.500.14299/26230
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