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research article

On-chip generation and detection of electrochemiluminescence

Fiaccabrino, G. C.
•
de Rooij, N. F.  
•
Koudelka-Hep, M.
1998
Analytica Chimica Acta

A miniaturized and fully integrated probe for analytical assays based on electrochemiluminescence (ECL) is proposed. It combines both, the electrode transducer and the photodetector in a single 5x6 mm2 silicon chip. The device accommodates two identical cells, each consisting of a gold interdigitated microelectrode array (IDA) resting on top of a pn photodiode, and of which one is used as an active cell and the other as a dummy. Light- intensity measurements are taken in a differential mode to increase the overall system performances. The device was characterized using ruthenium (II) tris(2,2'-bipyridyl) and tripropylamine as model system. The influence of both, the pH and the electrode to electrode distance on the response was investigated. The highest signal intensifies were detected in a phosphate buffer solution of pH 7.5 and an IDA gap of 0.8 μm. Excellent correlation was achieved between the peak light intensity and the concentration of the ruthenium complex over a 0.5-50 μM concentration range.

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Type
research article
DOI
10.1016/S0003-2670(97)00689-2
Author(s)
Fiaccabrino, G. C.
de Rooij, N. F.  
Koudelka-Hep, M.
Date Issued

1998

Published in
Analytica Chimica Acta
Volume

359

Issue

3

Start page

263

End page

267

Note

188

Editorial or Peer reviewed

REVIEWED

Written at

OTHER

EPFL units
SAMLAB  
Available on Infoscience
May 12, 2009
Use this identifier to reference this record
https://infoscience.epfl.ch/handle/20.500.14299/39242
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