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research article

A rivet model for channel formation by aerolysin-like pore-forming toxins

Iacovache, I.  
•
Paumard, P.
•
Scheib, H.
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2006
EMBO Journal

The bacterial toxin aerolysin kills cells by forming heptameric channels, of unknown structure, in the plasma membrane. Using disulfide trapping and cysteine scanning mutagenesis coupled to thiol-specific labeling on lipid bilayers, we identify a loop that lines the channel. This loop has an alternating pattern of charged and uncharged residues, suggesting that the transmembrane region has a beta-barrel configuration, as observed for Staphylococcal alpha-toxin. Surprisingly, we found that the turn of the beta-hairpin is composed of a stretch of five hydrophobic residues. We show that this hydrophobic turn drives membrane insertion of the developing channel and propose that, once the lipid bilayer has been crossed, it folds back parallel to the plane of the membrane in a rivet-like fashion. This rivet-like conformation was modeled and sequence alignments suggest that such channel riveting may operate for many other pore-forming toxins.

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Type
research article
DOI
10.1038/sj.emboj.7600959
Author(s)
Iacovache, I.  
•
Paumard, P.
•
Scheib, H.
•
Lesieur, C.
•
Sakai, N.
•
Matile, S.
•
Parker, M. W.
•
van der Goot, F. G.  
Date Issued

2006

Published in
EMBO Journal
Volume

25

Issue

3

Start page

457

End page

66

Subjects

Amino Acid Sequence

•

Animals

•

Bacterial Toxins/*chemistry/genetics/metabolism

•

Cell Line

•

Cell Membrane/*chemistry/metabolism

•

Cricetinae

•

Cysteine/chemistry

•

Ion Channels/metabolism

•

Lipid Bilayers/chemistry

•

*Models

•

Molecular

•

Molecular Sequence Data

•

Mutation

•

Pore Forming Cytotoxic Proteins

•

Protein Binding

•

Protein Conformation

Note

Department of Microbiology and Molecular Medicine, University of Geneva, Geneva, Switzerland.

Peer reviewed

REVIEWED

Written at

OTHER

EPFL units
VDG  
Available on Infoscience
January 30, 2009
Use this identifier to reference this record
https://infoscience.epfl.ch/handle/20.500.14299/34644
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