Biomolecule analysis by fluorescence intermittence signal recognition
The invention relates to a method for identifying a variant of a biomolecule among a plurality of variants of the biomolecule by these steps: A) Acquiring a fluorescence intermittence signal pattern for each variant. Each of the variants is labelled with a blinking fluorophore. B) Determining a fluorescence intermittency signal characteristic for each variant. C) Labelling at least one variant of the biomolecule in a sample with the fluorophore, and acquiring its fluorescence intermittency signal. D) Determining a presence of at least one fluorescence intermittency signal characteristic as determined in step B) in the acquired fluorescence intermittency signals from the sample. E) Establishing the presence, the quantity and/or localisation, of at least one variant of the biomolecule in the sample based on the fluorescence intermittency signal characteristic.
84366942
Alternative title(s) : (de) Biomolekülanalyse mittels erkennung von fluoreszenzinterminitätssignalen (fr) Analyse biomoleculaire par reconnaissance de signal d'intermite de fluorescence
| Identifier | Country code | Kind code | Date issued |
EP4379383 | EP | A1 | 2024-06-05 |