Repository logo

Infoscience

  • English
  • French
Log In
Logo EPFL, École polytechnique fédérale de Lausanne

Infoscience

  • English
  • French
Log In
  1. Home
  2. Academic and Research Output
  3. Journal articles
  4. An out-of-equilibrium definition of protein turnover
 
research article

An out-of-equilibrium definition of protein turnover

Martin, Benjamin  
•
Suter, David M.  
March 30, 2023
Bioessays

Protein turnover (PT) has been formally defined only in equilibrium conditions, which is ill-suited to quantify PT during dynamic processes that occur during embryogenesis or (extra) cellular signaling. In this Hypothesis, we propose a definition of PT in an out-of-equilibrium regime that allows the quantification of PT in virtually any biological context. We propose a simple mathematical and conceptual framework applicable to a broad range of available data, such as RNA sequencing coupled with pulsed-SILAC datasets. We apply our framework to a published dataset and show that stimulation of mouse dendritic cells with LPS leads to a proteome-wide change in PT. This is the first quantification of PT out-of-equilibrium, paving the way for the analysis of biological systems in other contexts.

  • Details
  • Metrics
Type
research article
DOI
10.1002/bies.202200209
Web of Science ID

WOS:000961554400001

Author(s)
Martin, Benjamin  
Suter, David M.  
Date Issued

2023-03-30

Published in
Bioessays
Subjects

Biochemistry & Molecular Biology

•

Biology

•

Biochemistry & Molecular Biology

•

Life Sciences & Biomedicine - Other Topics

•

dynamics

•

flux analysis

•

gene expression

•

out-of-equilibrium

•

protein turnover

•

rna-seq

•

silac

•

newly synthesized proteins

•

mass-spectrometry

•

cell-culture

•

proteomic analysis

•

amino-acids

•

dynamics

•

reveals

•

degradation

•

phosphorylation

•

quantification

Editorial or Peer reviewed

REVIEWED

Written at

EPFL

Available on Infoscience
April 24, 2023
Use this identifier to reference this record
https://infoscience.epfl.ch/handle/20.500.14299/197042
Logo EPFL, École polytechnique fédérale de Lausanne
  • Contact
  • infoscience@epfl.ch

  • Follow us on Facebook
  • Follow us on Instagram
  • Follow us on LinkedIn
  • Follow us on X
  • Follow us on Youtube
AccessibilityLegal noticePrivacy policyCookie settingsEnd User AgreementGet helpFeedback

Infoscience is a service managed and provided by the Library and IT Services of EPFL. © EPFL, tous droits réservés