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  4. Improved growth and methane production conditions for Methanobacterium thermoautotrophicum
 
conference paper

Improved growth and methane production conditions for Methanobacterium thermoautotrophicum

Gerhard, Elke
•
Butsch, Bertram M.
•
Marison, Ian
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1990
Proc. - Eur. Congr. Biotechnol., 5th

M. thermoautotrophicum was grown on a defined mineral salts medium under strictly anaerobic conditions with H2 and CO2 as the sole energy and C source, resp. The cultivation medium was optimized with respect to inorg. components, including Se(IV), W(VI), N, Ni(II), Fe(II), Co(II), and Mo(VI). S2- concn. in the medium was maintained const. using an online regulatory system by the addn. of 0.5M Na2S. A max. supply rate of 0.6 VVM of a mixt. of 80% H2 and 20% CO2 was achieved for the gaseous substrates. Under these conditions, the max. sp. growth rate (m) was 0.30/h and the cell concn. reached 4.8 g cell dry wt./L, representing a 140% increase over previously published results, in <20 h. At the end of the culture, the prodn. of CH4 was stationary at 0.09 VVM, corresponding to a substrate conversion of 75%. [on SciFinder (R)]

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Type
conference paper
Author(s)
Gerhard, Elke
Butsch, Bertram M.
Marison, Ian
Von Stockar, Urs  
Date Issued

1990

Published in
Proc. - Eur. Congr. Biotechnol., 5th
Volume

1

Start page

336

End page

8

Editorial or Peer reviewed

REVIEWED

Written at

EPFL

EPFL units
LGCB  
Available on Infoscience
February 27, 2006
Use this identifier to reference this record
https://infoscience.epfl.ch/handle/20.500.14299/225956
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