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  4. On-Chip Spyhole Nanoelectrospray Ionization Mass Spectrometry for Sensitive Biomarker Detection in Small Volumes
 
research article

On-Chip Spyhole Nanoelectrospray Ionization Mass Spectrometry for Sensitive Biomarker Detection in Small Volumes

Zhong, Xiaoqin  
•
Qiao, Liang
•
Stauffer, Géraldine
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March 5, 2018
Journal of The American Society for Mass Spectrometry

A polyimide microfluidic chip with a microhole emitter (Ø 10–12 μm) created on top of a microchannel by scanning laser ablation has been designed for nanoelectrospray ionization (spyhole-nanoESI) to couple microfluidics with mass spectrometry. The spyhole-nanoESI showed higher sensitivity compared to standard ESI and microESI from the end of the microchannel. The limits of detection (LOD) for peptide with the spyhole-nanoESI MS reached 50 pM, which was 600 times lower than that with standard ESI. The present microchip emitter allows the analysis of small volumes of samples. As an example, a small cell lung cancer biomarker, neuron-specific enolase (NSE), was detected by monitoring the transition of its unique peptide with the spyhole-nanoESI MS/MS. NSE at 0.2 nM could be well identified with a signal to noise ratio (S/N) of 50, and thereby its LOD was estimated to be 12 pM. The potential application of the spyhole-nanoESI MS/MS in cancer diagnosis was further demonstrated with the successful detection of 2 nMNSE from1 μL of human serum. Before the detection, the serumsample spiked with NSE was first depleted with immune spin column, then desalted by centrifugal filter device, and finally digested by trypsin, without any other complicated preparation steps. The concentration matched the real condition of clinical samples. In addition, the microchips can be disposable to avoid any cross contamination. The present technique provides a highly efficient way to couple microfluidics with MS, which brings additional values to various microfluidics and MS-based analysis.

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Type
research article
DOI
10.1007/s13361-018-1937-7
Author(s)
Zhong, Xiaoqin  
Qiao, Liang
Stauffer, Géraldine
Liu, Baohong
Girault, Hubert H.
Date Issued

2018-03-05

Published in
Journal of The American Society for Mass Spectrometry
Volume

29

Issue

7

Start page

1538

End page

1545

Editorial or Peer reviewed

REVIEWED

Written at

EPFL

EPFL units
LEPA  
Available on Infoscience
March 30, 2018
Use this identifier to reference this record
https://infoscience.epfl.ch/handle/20.500.14299/145834
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