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  4. Two-photon fluorescence imaging through multicore fiber with digital phase conjugation
 
conference paper

Two-photon fluorescence imaging through multicore fiber with digital phase conjugation

Stasio, Nicolino  
•
Conkey, Donald B.
•
Moser, Christophe
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Bifano, Tg
•
Kubby, J
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2016
Adaptive Optics And Wavefront Control For Biological Systems Ii
Conference on Adaptive Optics and Wavefront Control for Biological Systems II

We present near diffraction limited two photon fluorescence (TPF) imaging through a lensless, multi-core fiber (MCF) endoscope utilizing digital phase conjugation. The ultra-small size of MCFs make them desirable tools for imaging deep into the body. TPF imaging enables optical sectioning and is widely used in brain and biological imaging and is a desired modality for fiber endoscopes. Previous implementations of TPF imaging through MCFs focus and scan the light from individual cores for image formation. In such systems the resolution is limited by the MCF core spacing, although a lens may be used to improve the resolution at the expense of the field of view. Other, more recent work has improved the resolution limitation using custom built MCFs for focusing and scanning of ultrafast pulses using wavefront shaping. Here we present digital phase conjugation for ultrafast pulse focusing through a MCF for an imaging resolution independent of the MCF core spacing. Furthermore, the phase conjugation technique does not require the use of a lens at the fiber end for focus formation and is compatible with commercially available MCFs with a large number of cores. Here, we present a 3000 core MCF endoscope and demonstrate ultrafast pulse focusing with sufficient focus spot contrast and power for TPF endoscope imaging. We construct TPF images by digital scanning of the phase conjugated focus on the target object and collection of the emitted fluorescence through the MCF. This work demonstrates the viability of digital conjugation combined with commercially available MCFs for higher resolution lensless, two photon endoscopy.

  • Details
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Type
conference paper
DOI
10.1117/12.2212828
Web of Science ID

WOS:000389085900042

Author(s)
Stasio, Nicolino  
Conkey, Donald B.
Moser, Christophe
Psaltis, Demetri  
Editors
Bifano, Tg
•
Kubby, J
•
Gigan, S
Date Issued

2016

Publisher

Spie-Int Soc Optical Engineering

Publisher place

Bellingham

Published in
Adaptive Optics And Wavefront Control For Biological Systems Ii
ISBN of the book

978-1-62841-951-1

Total of pages

5

Series title/Series vol.

Proceedings of SPIE

Volume

9717

Start page

97171F

Subjects

endoscope

•

ultrafast pulse

•

two photon fluorescence

•

phase conjugation

•

multicore fiber

Editorial or Peer reviewed

REVIEWED

Written at

EPFL

EPFL units
LO  
Event nameEvent placeEvent date
Conference on Adaptive Optics and Wavefront Control for Biological Systems II

San Francisco, CA

FEB 13-15, 2016

Available on Infoscience
January 24, 2017
Use this identifier to reference this record
https://infoscience.epfl.ch/handle/20.500.14299/133351
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