Publication:

Comparison between SOFI and STORM

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Sayed, Ali H.

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Sayed, Ali H.

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datacite.rights

openaccess

dc.contributor.author

Geissbühler, Stefan

dc.contributor.author

Dellagiacoma, Claudio

dc.contributor.author

Lasser, Theo

dc.date.accessioned

2011-02-02T10:31:53

dc.date.available

2011-02-02T10:31:53

dc.date.created

2011-02-02

dc.date.issued

2011

dc.date.modified

2024-10-18T06:35:24.036157Z

dc.description.abstract

A straightforward method to achieve super-resolution consists of taking an image sequence of stochastically blinking emitters using a standard wide-field fluorescence microscope. Densely packed single molecules can be distinguished sequentially in time using high-precision localization algorithms (e.g., PALM and STORM) or by analyzing the statistics of the temporal fluctuations (SOFI). In a face-to-face comparison of the two post-processing algorithms, we show that localization-based super-resolution can deliver higher resolution enhancements but imposes significant constraints on the blinking behavior of the probes, which limits its applicability for live-cell imaging. SOFI, on the other hand, works more consistently over different photo-switching kinetics and also delivers information about the specific blinking statistics. Its suitability for low SNR acquisition reveals SOFI's potential as a high-speed super-resolution imaging technique.

dc.description.notes

This paper was published in Biomedical Optics Express and is made available as an electronic reprint with the permission of OSA. The paper can be found at the following URL on the OSA website: http://www.opticsinfobase.org/boe/abstract.cfm?uri=boe-2-3-408. Systematic or multiple reproduction or distribution to multiple locations via electronic or other means is prohibited and is subject to penalties under law.

dc.description.sponsorship

LOB

dc.identifier.doi

10.1364/BOE.2.000408

dc.identifier.isi

WOS:000299875200001

dc.identifier.uri

https://infoscience.epfl.ch/handle/20.500.14299/63856

dc.relation

https://infoscience.epfl.ch/record/163023/files/sofivsstorm.pdf

dc.relation.issn

2156-7085

dc.relation.journal

Biomedical Optics Express

dc.subject

Superresolution

dc.subject

Fluorescence microscopy

dc.title

Comparison between SOFI and STORM

dc.type

text::journal::journal article::research article

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Publication

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oai:infoscience.tind.io:163023

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n/a

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Journal Articles

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ARTICLE

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OpenAIREv4

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STI

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article

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REVIEWED

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http://purl.org/coar/version/c_970fb48d4fbd8a85

epfl.writtenAt

EPFL

oaire.citation.endPage

420

oaire.citation.issue

3

oaire.citation.startPage

408

oaire.citation.volume

2

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