Repository logo

Infoscience

  • English
  • French
Log In
Logo EPFL, École polytechnique fédérale de Lausanne

Infoscience

  • English
  • French
Log In
  1. Home
  2. Academic and Research Output
  3. Conferences, Workshops, Symposiums, and Seminars
  4. Optimizing the extraction of extracellular DNA of aerobic granular biofilms
 
conference poster not in proceedings

Optimizing the extraction of extracellular DNA of aerobic granular biofilms

Bialek, Katarzyna  
2016
Swiss Society for Microbiology

Formation and properties of aerobic granules for enhanced biological phosphorus removal (EBPR) from sequencing batch reactor (SBR) strongly depend on the extracellular polymeric substances (EPS) in which the microorganisms are embedded. The composition of EPS varies between different species but mostly it is made up of proteins, polysaccharides and extracellular DNA (eDNA), that has been recently recognized as an important structural component. Although great amount of literature on the EPS extraction is available, till now, there is no standardized protocol for EPS extraction. Extraction methods involving multi step processes seem to be the most promising, although too harsh extraction procedures can lyse the cells and release intracellular compounds, which contaminate the EPS samples creating bias. Commonly employed intracellular markers of cell lysis i.e. Glucose-6-phosphate dehydrogenase (G6PDH) or 2-keto-3deoxyoctonate (KDO) are not always applicable, especially in systems where glycolysis does not occur and in mixed communities with gram-positive bacteria, respectively. Therefore a new method to measure cell lysis is needed. The activity of isocitrate dehydrogenase (ICDH), an ubiquitous enzyme found in all living organisms utilzing the Krebs (citric acid) cycle, has been assessed. Initial assays using this enzyme activity during EPS extraction revealed to be very sensitive to detect cell lysis in samples from mixed microbial communities as granular sludge biofilms cultivated in SBR. Preliminary results of EPS extraction using multi-step method and enzymatic extraction as well as selection of appropriate dyes to visualize and distinguish intracellular and extracellular DNA in granular samples from the reactor are discussed.

  • Details
  • Metrics
Type
conference poster not in proceedings
Author(s)
Bialek, Katarzyna  
Date Issued

2016

Written at

EPFL

EPFL units
LBE  
Event nameEvent placeEvent date
Swiss Society for Microbiology

Bern, Switzerland

June 13-15, 2016

Available on Infoscience
January 18, 2017
Use this identifier to reference this record
https://infoscience.epfl.ch/handle/20.500.14299/132980
Logo EPFL, École polytechnique fédérale de Lausanne
  • Contact
  • infoscience@epfl.ch

  • Follow us on Facebook
  • Follow us on Instagram
  • Follow us on LinkedIn
  • Follow us on X
  • Follow us on Youtube
AccessibilityLegal noticePrivacy policyCookie settingsEnd User AgreementGet helpFeedback

Infoscience is a service managed and provided by the Library and IT Services of EPFL. © EPFL, tous droits réservés