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research article

Off-axis digital holographic camera for quantitative phase microscopy

Monemhaghdoust, Zahra  
•
Montfort, Frederic
•
Emery, Yves
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2014
Biomedical Optics Express

We propose and experimentally demonstrate a digital holographic camera which can be attached to the camera port of a conventional microscope for obtaining digital holograms in a self-reference configuration, under short coherence illumination and in a single shot. A thick holographic grating filters the beam containing the sample information in two dimensions through diffraction. The filtered beam creates the reference arm of the interferometer. The spatial filtering method, based on the high angular selectivity of the thick grating, reduces the alignment sensitivity to angular displacements compared with pinhole based Fourier filtering. The addition of a thin holographic grating alters the coherence plane tilt introduced by the thick grating so as to create high-visibility interference over the entire field of view. The acquired full-field off-axis holograms are processed to retrieve the amplitude and phase information of the sample. The system produces phase images of cheek cells qualitatively similar to phase images extracted with a standard commercial DHM. (C) 2014 Optical Society of America

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Type
research article
DOI
10.1364/Boe.5.001721
Web of Science ID

WOS:000337503900003

Author(s)
Monemhaghdoust, Zahra  
•
Montfort, Frederic
•
Emery, Yves
•
Depeursinge, Christian  
•
Moser, Christophe  
Date Issued

2014

Publisher

Optical Soc Amer

Published in
Biomedical Optics Express
Volume

5

Issue

6

Start page

1721

End page

1730

Peer reviewed

REVIEWED

Written at

EPFL

EPFL units
LOA  
LAPD  
Available on Infoscience
August 29, 2014
Use this identifier to reference this record
https://infoscience.epfl.ch/handle/20.500.14299/106499
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