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  4. Modulation of the endogenous production of protoporphyrin IX in a yeast - based model organism
 
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conference paper

Modulation of the endogenous production of protoporphyrin IX in a yeast - based model organism

Joniova, Jaroslava
•
Gerelli, Emmanuel  
•
Wagnieres, Georges  
Alfano, Rr
•
Demos, Sg
2017
Optical Biopsy Xv: Toward Real-Time Spectroscopic Imaging And Diagnosis
Conference on Optical Biopsy XV - Toward Real-Time Spectroscopic Imaging and Diagnosis

The main aim of this study was to assess conditions at which simple yeast-based model organism produces maximal levels of protoporphyrin IX (PpIX) after an exogenous administration of its precursor, 5-aminolevulinic acid (ALA), and the ferrous-ion chelator 2,2'-bipyridyl. We observed that the fluorescing porphyrin, produced after these administrations, was likely to be PpIX since fluorescence spectroscopy of the porphyrins produced endogenously in yeast cells resembles that of PpIX in DMSO and in vivo in the chick's chorioallantoic membrane model. Also, fluorescence lifetimes of these porphyrins are very similar to that of PpIX in vitro and in vivo. This suggests that PpIX is the main fluorescent compound produced by yeast in our conditions. We found that the conditions at which yeast produces the maximal PpIX were a synchronous administration of 5 mu M ALA and 1 mM 2,2'-bipyridyl for yeast incubated in aqueous glucose and 1 mM 2,2'-bipyridyl in the presence of YPD medium. Such a simple model is of high interest to study basic mechanisms involved in the mitochondrial respiration since PpIX, which is produced in this organelle, can be used as an oxygen sensor, or to perform photodynamic therapy and photodiagnosis. Since the absorption and scattering coefficients of this model are much smaller than those of soft tissues over the visible part of the spectrum, a version of this model loaded with appropriated amounts of light absorbing and scattering particles could be designed as a phantom to mimic tumors containing PpIX, a useful tool to optimize certain cancer photodetection set-ups.

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Type
conference paper
DOI
10.1117/12.2256504
Web of Science ID

WOS:000405952000029

Author(s)
Joniova, Jaroslava
•
Gerelli, Emmanuel  
•
Wagnieres, Georges  
Editors
Alfano, Rr
•
Demos, Sg
Date Issued

2017

Publisher

Spie-Int Soc Optical Engineering

Publisher place

Bellingham

Published in
Optical Biopsy Xv: Toward Real-Time Spectroscopic Imaging And Diagnosis
ISBN of the book

978-1-5106-0561-9

978-1-5106-0562-6

Total of pages

8

Series title/Series vol.

Proceedings of SPIE

Volume

10060

Start page

1006018

Subjects

model organism

•

endogenous protoporphyrin IX

•

optical phantom

•

PpIX spectroscopy

Peer reviewed

REVIEWED

Written at

EPFL

EPFL units
GPM  
Event nameEvent placeEvent date
Conference on Optical Biopsy XV - Toward Real-Time Spectroscopic Imaging and Diagnosis

San Francisco, CA

JAN 31-FEB 01, 2017

Available on Infoscience
September 5, 2017
Use this identifier to reference this record
https://infoscience.epfl.ch/handle/20.500.14299/140367
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