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  4. Preparation of 2D Crystals of Membrane Proteins for High-Resolution Electron Crystallography Data Collection
 
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Preparation of 2D Crystals of Membrane Proteins for High-Resolution Electron Crystallography Data Collection

Abeyrathne, Priyanka D.
•
Chami, Mohamed
•
Pantelic, Radosav S.
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January 1, 2010
Cryo-EM Part A Sample Preparation and Data Collection

Electron crystallography is a powerful technique for the structure determination of membrane proteins as well as soluble proteins. Sample preparation for 2D membrane protein crystals is a crucial step, as proteins have to be prepared for electron microscopy at close to native conditions. In this review, we discuss the factors of sample preparation that are key to elucidating the atomic structure of membrane proteins using electron crystallography.

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Type
book part or chapter
DOI
10.1016/S0076-6879(10)81001-8
Author(s)
Abeyrathne, Priyanka D.
Chami, Mohamed
Pantelic, Radosav S.
Goldie, Kenneth N.
Stahlberg, Henning  orcid-logo
Date Issued

2010-01-01

Publisher

Elsevier

Published in
Cryo-EM Part A Sample Preparation and Data Collection
ISBN of the book

978-0-12-374906-2

Start page

25

End page

43

Volume
481
Editorial or Peer reviewed

REVIEWED

Written at

OTHER

EPFL units
LBEM  
Available on Infoscience
February 13, 2020
Use this identifier to reference this record
https://infoscience.epfl.ch/handle/20.500.14299/165370
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