Chidley, ChristopherHaruki, HirohitoPedersen, Miriam GrønlundFellay, CindyMoser, SimoneJohnsson, Kai2011-12-162011-12-162011-12-16201110.2533/chimia.2011.720https://infoscience.epfl.ch/handle/20.500.14299/73447WOS:000295811300021The identification of all protein targets of a given drug or bioactive molecule within the human body is a prerequisite for an understanding of its beneficial and deleterious activities. Current approaches to reveal protein targets often fail to reveal physiologically relevant interactions. Here we review a recently introduced yeast-based approach for the identification of the binding partners of small molecules. We discuss the advantages and limitations of the approach using the clinically approved drug sulfasalazine as an example.Anti-Inflammatory Agents, Non-SteroidalSmall Molecule LibrariesSulfasalazineTarget deconvolutionTetrahydrobiopterinYeast three-hybrid screeningNitric-Oxide SynthaseDrug DiscoveryIn-VivoTetrahydrobiopterin BiosynthesisRheumatoid-ArthritisUlcerative-ColitisSulfasalazineInhibitionYeastMetabolomicstext::journal::journal article::research article