Panwar, JatinUtharala, RameshFennelly, LauraFrenzel, DanielMerten, Christoph A.2023-07-312023-07-312023-07-312023-05-2210.1016/j.crmeth.2023.100478https://infoscience.epfl.ch/handle/20.500.14299/199454WOS:001011745900001Fluorescence-activated droplet sorting (FADS) is a widely used microfluidic technique for high-throughput screening. However, it requires highly trained specialists to determine optimal sorting parameters, and this results in a large combinatorial space that is challenging to optimize systematically. Additionally, it is currently challenging to track every single droplet within a screen, leading to compromised sorting and "hid-den"false-positive events. To overcome these limitations, we have developed a setup in which the droplet frequency, spacing, and trajectory at the sorting junction are monitored in real time using impedance anal-ysis. The resulting data are used to continuously optimize all parameters automatically and to counteract perturbations, resulting in higher throughput, higher reproducibility, increased robustness, and a beginner-friendly character. We believe this provides a missing piece for the spreading of phenotypic sin-gle-cell analysis methods, similar to what we have seen for single-cell genomics platforms.Biochemical Research MethodsCell BiologyBiochemistry & Molecular Biologyhigh-throughputcell analysislabel-freesinglecytometrychiptext::journal::journal article::research article