Garcia-Cordero, Jose L.Maerkl, Sebastian J.2013-03-282013-03-282013-03-28201310.1039/c2cc37740chttps://infoscience.epfl.ch/handle/20.500.14299/90977WOS:000313663900003We report the flow-based in situ patterning of multiple proteins in microfluidic channels by simply tuning the actuation pressure of a microfluidic button-membrane. We show that a single button-membrane can pattern several concentric protein annuli, and we apply this patterning approach to a multiplexed immunoassay.text::journal::journal article::research article