Bubeck, FelixHoffmann, Mareike D.Harteveld, ZanderAschenbrenner, SabineBietz, AndreasWaldhauer, Max C.Boerner, KathleenFakhiri, JuliaSchmelas, CarolinDietz, LauraGrimm, DirkCorreia, Bruno E.Eils, RolandNiopek, Dominik2018-12-132018-12-132018-12-132018-11-0110.1038/s41592-018-0178-9https://infoscience.epfl.ch/handle/20.500.14299/152750WOS:000448827300028Anti-CRISPR proteins are powerful tools for CRISPR-Cas9 regulation; the ability to precisely modulate their activity could facilitate spatiotemporally confined genome perturbations and uncover fundamental aspects of CRISPR biology. We engineered optogenetic anti-CRISPR variants comprising hybrids of AcrIIA4, a potent Streptococcus pyogenes Cas9 inhibitor, and the LOV2 photosensor from Avena sativa. Coexpression of these proteins with CRISPR-Cas9 effectors enabled light-mediated genome and epigenome editing, and revealed rapid Cas9 genome targeting in human cells.Biochemical Research MethodsBiochemistry & Molecular Biologyloop closureinhibitioncellscas9acriia4text::journal::journal article::research article