Cuche, E.Marquet, P.Depeursinge, C.2009-07-202009-07-202009-07-20199910.1364/AO.38.006994https://infoscience.epfl.ch/handle/20.500.14299/41493WOS:000084023500003We present a digital method for holographic microscopy involving a CCD camera as a recording device. Off-axis holograms recorded with a magnified image of microscopic objects are numerically reconstructed in amplitude and phase by calculation of scalar diffraction in the Fresnel approximation. For phase-contrast imaging the reconstruction method involves the computation of a digital replica of the reference wave. A digital method for the correction of the phase aberrations is presented. We present a detailed description of the reconstruction procedure and show that the transverse resolution is equal to the diffraction limit of the imaging system.[MVD]Detectors : CCD, charge-coupled deviceHolography : HolographyHolography : Aberration compensationImaging systems : MicroscopyOther areas of optics : Phasetext::journal::journal article::research article