Bojkowska, KarolinaSantoni De Sio, FrancescaBarde, IsabelleOffner, SandraVerp, SoniaHeinis, ChristianJohnsson, KaiTrono, Didier2011-06-292011-06-292011-06-29201110.1016/j.chembiol.2011.03.014https://infoscience.epfl.ch/handle/20.500.14299/69067WOS:000292583800014Protein turnover critically influences many biological functions, yet methods have been lacking to assess this parameter in vivo. Here, we demonstrate how chemical labeling of SNAP-tag fusion proteins can be exploited to measure the half-life of resident intracellular and extracellular proteins in living mice. First, we demonstrate that SNAP-tag substrates have wide bioavailability in mice and can be used for the specific in vivo labeling of SNAP-tag fusion proteins. We then apply near-infrared probes to perform noninvasive imaging of in vivo-labeled tumors. Finally, we use SNAP-mediated chemical pulse-chase labeling to perform measurement of the in vivo half-life of different extra- and intracellular proteins. These results open broad perspectives for studying protein function in living animals.Tag Fusion ProteinsO-6-Alkylguanine-Dna AlkyltransferaseLentiviral VectorsLiving CellsCd4O-6-BenzylguanineExpressionUbiquitinSuperresolutionDegradationtext::journal::journal article::research article