Complete microscale profiling of tumor microangiogenesis A microradiological methodology reveals fundamental aspects of tumor angiogenesis and yields an array of quantitative parameters for its characterization

Chien, Chia-ChiKempson, Ivan M.Wang, Cheng LiangChen, H. H.Hwu, YeukuangChen, N. Y.Lee, T. K.Tsai, Kelvin K.-C.Liu, Ming-ShengChang, Kwang-YuYang, C. S.Margaritondo, G.2013-10-012013-10-012013-10-01201310.1016/j.biotechadv.2011.12.001https://infoscience.epfl.ch/handle/20.500.14299/95467WOS:000317441900007Complete profiling would substantially facilitate the fundamental understanding of tumor angiogenesis and of possible anti-angiogenesis cancer treatments. We developed an integrated synchrotron-based methodology with excellent performances: detection of very small vessels by high spatial resolution (-1 mu m) and nanoparticle contrast enhancement, in vivo dynamics investigations with high temporal resolution (-1 ms), and three-dimensional quantitative morphology parametrization by computer tracing. The smallest (3-10 mu m) microvessels were found to constitute >80% of the tumor vasculature and exhibit many structural anomalies. Practical applications are presented, including vessel microanalysis in xenografted tumors, monitoring the effects of anti-angiogenetic agents and in vivo detection of tumor vascular rheological properties. (C) 2011 Elsevier Inc. All rights reserved.AngiogenesisSynchrotronX-ray imagingSolid tumorQuantitative analysisComplete microscale profiling of tumor microangiogenesis A microradiological methodology reveals fundamental aspects of tumor angiogenesis and yields an array of quantitative parameters for its characterizationtext::conference output::conference proceedings::conference paper