Künzi, P. A.Lussi, J.Aeschimann, L.Danuser, G.Textor, M.de Rooij, N. F.Staufer, U.2009-05-122009-05-122009-05-12200510.1016/j.mee.2004.12.073https://infoscience.epfl.ch/handle/20.500.14299/39504A method for fabricating sub-micrometer size adhesion sites for future experiments in cell biology is presented. Glass substrates were coated with a thin layer of InSnO and SiO2. The SiO2 was structured by means of electron beam lithography and reactive ion etching, exposing sub-micrometer patches of the underlying InSnO. Dodecylphosphate, to which proteins can bind, was selectively adsorbed on these InSnO structures, whereas poly-l-lysine-g-poly(ethylene glycol) was used to passivate the surrounding SiO2 against protein adsorption. The effectiveness of the process was investigated by fluorescent microscopy and scanning near-field optical microscopy on substrates which have been exposed to fluorescently labeled streptavidin. © 2005 Elsevier B.V. All rights reserved.text::journal::journal article::research article