Geissbühler, StefanAguet, FrançoisMärki, IwanLasser, Theo2010-03-122010-03-122010-03-12201010.1117/12.841783https://infoscience.epfl.ch/handle/20.500.14299/48051WOS:000284353600013The diffraction patterns of fixed fluorophores are characteristic of the orientation of the molecules' underlying dipole. Fluorescence localization microscopy techniques such as PALM and STORM achieve super-resolution by sequentially imaging sparse subsets of fluorophores, which are localized by means of Gaussian-based localization. This approach is based on the assumption of isotropic emitters, where the diffraction pattern corresponds to a section of the point spread function. Applied to fixed fluorophores, it can lead to an estimation bias in the range of 5-20nm. We introduce a method for the joint estimation of position and orientation of single fluorophores, based on an accurate image formation model expressed as a 3-D steerable filter. We demonstrate experimental estimation accuracies of 5 nm for position and 2 degrees for orientation.Super-resolutiondipole imagingsingle moleculeorientation estimationlocalizationsteerable flterstext::conference output::conference proceedings::conference paper