Sieben, ChristianBanterle, NiccoloGönczy, PierreDouglass, Kyle M.Manley, Suliana2018-12-132018-12-132018-12-132018-10-0110.1038/s41592-018-0140-xhttps://infoscience.epfl.ch/handle/20.500.14299/152054WOS:000448820100022Single-particle reconstruction (SPR) from electron microscopy (EM) images is widely used in structural biology, but it lacks direct information on protein identity. To address this limitation, we developed a computational and analytical framework that reconstructs and coaligns multiple proteins from 2D super-resolution fluorescence images. To demonstrate our method, we generated multicolor 3D reconstructions of several proteins within the human centriole, which revealed their relative locations, dimensions and orientations.Biochemical Research MethodsBiochemistry & Molecular Biologysuperresolution microscopyelectron-microscopycryo-emarchitecturecentrosomesresolutionrevealscellspurificationilluminationMulticolor single-particle reconstruction of protein complexestext::journal::journal article::research article