Redox-cycling type electrochemiluminescence in aqueous medium. A new principle for the detection of proteins labeled with a ruthenium chelate

Michel, P.de Rooij, N. F.Koudelka-Hep, M.Faehnrich, K. A.O'Sullivan, C. K.Guilbault, G. G.2009-05-122009-05-122009-05-12199910.1016/S0022-0728(99)00351-4https://infoscience.epfl.ch/handle/20.500.14299/39606The first observation of electrochemiluminescence (ecl) based on the redox-cycling of a ruthenium chelate covalently linked to a protein and placed in aqueous medium containing neither solvent nor reducing compound is reported here. Ecl experiments based on redox-cycling were performed in phosphate buffer at carbon interdigitated microelectrode arrays. The light intensity represented 31% of that obtained using a non complexed Ru(bpy)3 2+ solution prepared in phosphate buffer containing proteins.Redox-cycling type electrochemiluminescence in aqueous medium. A new principle for the detection of proteins labeled with a ruthenium chelatetext::journal::journal article::research article