Tan, Cedric C. S.Trew, JahcubPeacock, Thomas P.Mok, Kai YiHart, CharlieLau, KelvinNi, DongchunOrme, C. David L.Ransome, EmmaPearse, William D.Coleman, Christopher M.Bailey, DalanThakur, NaziaQuantrill, Jessica L.Sukhova, KseniaRichard, DamienKahane, LauraWoodward, GuyBell, ThomasWorledge, LisaNunez-Mino, JoeBarclay, Wendyvan Dorp, LucyBalloux, FrancoisSavolainen, Vincent2023-08-282023-08-282023-08-282023-06-2710.1038/s41467-023-38717-whttps://infoscience.epfl.ch/handle/20.500.14299/200292WOS:001022863800011There has been limited characterisation of bat-borne coronaviruses in Europe. Here, we screened for coronaviruses in 48 faecal samples from 16 of the 17 bat species breeding in the UK, collected through a bat rehabilitation and conservationist network. We recovered nine complete genomes, including two novel coronavirus species, across six bat species: four alphacoronaviruses, a MERS-related betacoronavirus, and four closely related sarbecoviruses. We demonstrate that at least one of these sarbecoviruses can bind and use the human ACE2 receptor for infecting human cells, albeit suboptimally. Additionally, the spike proteins of these sarbecoviruses possess an R-A-K-Q motif, which lies only one nucleotide mutation away from a furin cleavage site (FCS) that enhances infectivity in other coronaviruses, including SARS-CoV-2. However, mutating this motif to an FCS does not enable spike cleavage. Overall, while UK sarbecoviruses would require further molecular adaptations to infect humans, their zoonotic risk warrants closer surveillance.Certain bats species have previously been identified as ancestral sources of coronaviruses that infect humans but there is limited data on the genomic diversity or zoonotic potential of viruses infecting bats in the UK. Here, the authors use deep sequencing and in vitro assays to characterise coronaviruses recovered from 48 bat faecal samples.Multidisciplinary SciencesScience & Technology - Other Topicsbindingvisualizationastrovirusesperformancealignmentplatformvirusesspikeace2text::journal::journal article::research article