Tobolkina, ElenaCortes Salazar, FernandoQiao, LiangGirault, Hubert H.2014-04-072014-04-072014-04-07201410.1039/C4AY00088Ahttps://infoscience.epfl.ch/handle/20.500.14299/102572WOS:000337121800013Purification and desalting of protein and peptide samples by a three-well OFFGEL electrophoresis with immobilized ultra narrow pH gradient gels is proposed as a fast preparative strategy for proteomics. The gist of this strategy is to separate the proteins and peptides according to their isoelectric point and to isolate those of a given pI value equal to the mean pH value of the gel. The present approach has been demonstrated both on protein mixtures and a digested Escherichia coli protein extract. UV-Vis spectroscopy, MALDI-MS, SDS-PAGE and LC-MS/MS were employed for the quantitative and qualitative characterization of the separation results. The electrophoretic methodology has been simulated by finite element methods.text::journal::journal article::research article