Boivin, GaelFaget, JulienAncey, Pierre-BenoitGkasti, AspasiaMussard, JulieEngblom, CamillaPfirschke, ChristinaContat, CarolinePascual, JustineVazquez, JessicaBendriss-Vermare, NathalieCaux, ChristopheVozenin, Marie-CatherinePittet, Mikael J.Gunzer, MatthiasMeylan, Etienne2020-07-162020-07-162020-07-162020-06-0210.1038/s41467-020-16596-9https://infoscience.epfl.ch/handle/20.500.14299/170177WOS:000543974800018Neutrophils are an essential part of the innate immune system. To study their importance, experimental studies often aim to deplete these cells, generally by injecting anti-Ly6G or anti-Gr1 antibodies. However, these approaches are only partially effective, transient or lack specificity. Here we report that neutrophils remaining after anti-Ly6G treatment are newly derived from the bone marrow, instead of depletion escapees. Mechanistically, newly generated, circulating neutrophils have lower Ly6G membrane expression, and consequently reduced targets for anti-Ly6G-mediated depletion. To overcome this limitation, we develop a double antibody-based depletion strategy that enhances neutrophil elimination by anti-Ly6G treatment. This approach achieves specific, durable and controlled reduction of neutrophils in vivo, and may be suitable for studying neutrophil function in experimental models.Multidisciplinary SciencesScience & Technology - Other Topicsbone-marrowt-cellsinfectionkineticstraffickingsurvivalantibodyrevealsmodeltext::journal::journal article::research article