Schuhmann, KaiSrzentic, KristinaNagornov, Konstantin O.Thomas, HenrikGutmann, TheresiaCoskun, UnalTsybin, Yury O.Shevchenko, Andrej2018-01-152018-01-152018-01-15201710.1021/acs.analchem.7b03437https://infoscience.epfl.ch/handle/20.500.14299/144055WOS:000417549600036Lipidomes undergo permanent extensive remodeling, but how the turnover rate differs between lipid classes and molecular species is poorly understood. We employed metabolic N-15 labeling and shotgun ultra-high-resolution mass spectrometry (sUHR) to quantify the absolute (molar) abundance and determine the turnover rate of glycerophospholipids and sphingolipids by direct analysis of total lipid extracts. sUHR performed on a commercial Orbitrap Elite instrument at the mass resolution of 1.35 x 10 (6) (m/z 200) baseline resolved peaks of C-13 isotopes of unlabeled and monoisotopic peaks of N-15 labeled lipids (Delta m = 0.0063 Da). Therefore, the rate of metabolic N-15 labeling of individual lipid species could be determined without compromising the scope, accuracy, and dynamic range of full-lipidome quantitative shotgun profiling. As a proof of concept, we employed sUHR to determine the lipidome composition and fluxes of 62 nitrogen-containing membrane lipids in human hepatoma HepG2 cells.text::journal::journal article::research article