De Jong, Christiaan J.Lajevardipour, AlirezaGecevicius, MindaugasBeresna, MartynasGervinskas, GediminasKazansky, Peter G.Bellouard, YvesClayton, Andrew H. A.Juodkazis, Saulius2016-02-162016-02-162016-02-16201510.1364/Prj.3.000283https://infoscience.epfl.ch/handle/20.500.14299/124202WOS:000366594800024A novel fluorescence lifetime imaging microscopy (FLIM) working with deep UV 240-280 nm wavelength excitations has been developed. UV-FLIM is used for measurement of defect-related fluorescence and its changes upon annealing from femtosecond laser-induced modifications in fused silica. This FLIM technique can be used with microfluidic and biosamples to characterize temporal characteristics of fluorescence upon UV excitation, a capability easily added to a standard microscope-based FLIM. UV-FLIM was tested to show annealing of the defects induced by silica structuring with ultrashort laser pulses. Frequency-domain fluorescence measurements were converted into the time domain to extract long fluorescence lifetimes from defects in silica. (C) 2015 Chinese Laser Presstext::journal::journal article::research article