Kühn, JonasMontfort, FrédéricColomb, TristanRappaz, BenjaminMoratal, CorinnePavillon, NicolasMarquet, PierreDepeursinge, Christian2009-07-202009-07-202009-07-20200910.1364/OL.34.000653https://infoscience.epfl.ch/handle/20.500.14299/41661WOS:000264522400035We present first results on a method enabling mechanical scanning-free tomography with submicrometer axial resolution by multiple-wavelength digital holographic microscopy. By sequentially acquiring reflection holograms and summing 20 wavefronts equally spaced in spatial frequency in the 485–670 nm range, we are able to achieve a slice-by-slice tomographic reconstruction with a 0.6–1 μm axial resolution in a biological medium. The method is applied to erythrocytes investigation to retrieve the cellular membrane profile in three dimensions.[MVD]MicroscopyTomographyDigital holographyTomographic imagingSubmicrometer tomography of cells by multiple-wavelength digital holographic microscopy in reflectiontext::journal::journal article::research article