Dénervaud, NicolasBecker, JohannesDelgado-Gonzalo, RicardDamay, PascalRajkumar, Arun S.Unser, MichaelShore, DavidNaef, FelixMaerkl, Sebastian J.2013-09-262013-09-262013-09-26201310.1073/pnas.1308265110https://infoscience.epfl.ch/handle/20.500.14299/94968WOS:000324765100071Observing cellular responses to perturbations is central to generating and testing hypotheses in biology. We developed a massively parallel microchemostat array capable of growing and observing 1,152 yeast-GFP strains on the single-cell level with 20 min time resolution. We measured protein abundance and localization changes in 4,085 GFP-tagged strains in response to methyl methanesulfonate and analyzed 576 GFP strains in five additional conditions for a total of more than 10,000 unique experiments, providing a systematic view of the yeast proteome in flux. We observed that processing bodies formed rapidly and synchronously in response to UV irradiation, and in conjunction with 506 deletion-GFP strains, identified four gene disruptions leading to abnormal ribonucleotide-diphosphate reductase (Rnr4) localization. Our microchemostat platform enables the large-scale interrogation of proteomes in flux and permits the concurrent observation of protein abundance, localization, cell size, and growth parameters on the single-cell level for thousands of microbial cultures in one experiment.microfluidicscell arrayshigh-content imagingDNA damage responseyeast proteomicstext::journal::journal article::research article