Joshi, OnkarWang, Shuang-YinKuznetsova, TatyanaAtlasi, YaserPeng, TianranFabre, Pierre J.Habibi, EhsanShaik, JaniSaeed, SadiaHandoko, LusyRichmond, ToddSpivakov, MikhailBurgess, DanielStunnenberg, Hendrik G.2016-02-162016-02-162016-02-16201510.1016/j.stem.2015.11.010https://infoscience.epfl.ch/handle/20.500.14299/123919WOS:000366139100015Serum-to-2i interconversion of mouse embryonic stem cells (mESCs) is a valuable in vitro model for early embryonic development. To assess whether 3D chromatin organization changes during this transition, we established Capture Hi-C with target-sequence enrichment of DNase I hypersensitive sites. We detected extremely long-range intra-and inter-chromosomal interactions between a small subset of H3K27me3 marked bivalent promoters involving the Hox clusters in serum-grown cells. Notably, these promoter-mediated interactions are not present in 2i ground-state pluripotent mESCs but appear upon their further development into primed-like serum mESCs. Reverting serum mESCs to ground-state 2i mESCs removes these promoter-promoter interactions in a spatiotemporal manner. H3K27me3, which is largely absent at bivalent promoters in ground-state 2i mESCs, is necessary, but not sufficient, to establish these interactions, as confirmed by Capture Hi-C on Eed(-/-) serum mESCs. Our results implicate H3K27me3 and PRC2 as critical players in chromatin alteration during priming of ESCs for differentiation.text::journal::journal article::research article