Tafelmeyer, PetraLaurent, ChristineLenormand, PascalRousselle, Jean-ClaudeMarsollier, LaurentReysset, GillesZhang, RunxuanSickmann, AlbertStinear, Timothy PNamane, AbdelkaderCole, Stewart T2010-09-072010-09-072010-09-07200810.1002/pmic.200701018https://infoscience.epfl.ch/handle/20.500.14299/53103WOS:00025850340001418615429Mycobacterium ulcerans is the causative agent of Buruli ulcer, a rapidly emerging human disease in which mycolactone, a cytotoxic and immunosuppressive macrocyclic polyketide, is responsible for massive skin destruction. The genome sequencing of M. ulcerans has recently been accomplished (http://genolist.pasteur.fr/BuruList/) enabling the first proteome study of this important human pathogen. Here, we present a comprehensive proteome analysis of different subcellular fractions and culture supernatant of in vitro grown M. ulcerans. By a combination of gel-based and gel-free techniques for protein and peptide separation with subsequent analysis by MS, we identified 1074 different proteins, corresponding to 25% of the protein-coding DNA sequence. Interestingly, new information was obtained about central metabolism and lipid biosynthesis, and as many as 192 conserved hypothetical proteins were found. Comparative analysis of the wild-type strain and an isogenic mycolactone-deficient mutant, by 2-DE and iTRAQ labeling of the cytoplasmic fraction, revealed differences in the expression profiles of proteins involved in lipid metabolism and information pathways, as well as stress responses.text::journal::journal article::research article