Soler-Arnedo, PalomaSala, ClaudiaZhang, MingCole, Stewart T.Piton, Jeremie2020-03-052020-03-052020-03-052020-03-0110.1128/JB.00662-19https://infoscience.epfl.ch/handle/20.500.14299/166997WOS:000513882300004Mycobacterium tuberculosis is a slow-growing intracellular bacterium with the ability to induce host cell death and persist indefinitely in the human body. This pathogen uses the specialized ESX-1 secretion system to secrete virulence factors and potent immunogenic effectors required for disease progression. ESX-1 is a multisubunit apparatus with a membrane complex that is predicted to form a channel in the cytoplasmic membrane. In M. tuberculosis this complex is composed of five membrane proteins: EccB(1), EccCa(1), EccCb(1), EccD(1), and EccE(1). In this study, we have characterized the membrane component EccE(1) and found that deletion of eccE(1) lowers the levels of EccB(1), EccCa(1), and EccD(1), thereby abolishing ESX-1 secretion and attenuating M. tuberculosis ex vivo. Surprisingly, secretion of EspB was not affected by loss of EccE(1). Furthermore, EccE(1) was found to be a membrane- and cell wall-associated protein that needs the presence of other ESX-1 components to assemble into a stable complex at the poles of M. tuberculosis. Overall, this investigation provides new insights into the role of EccE(1) and its localization in M. tuberculosis.IMPORTANCE Tuberculosis (TB), the world's leading cause of death of humans from an infectious disease, is caused by the intracellular bacterium Mycobacterium tuberculosis. The development of successful strategies to control TB requires better understanding of the complex interactions between the pathogen and the human host. We investigated the contribution of EccE(1), a membrane protein, to the function of the ESX-1 secretion system, the major virulence determinant of M. tuberculosis. By combining genetic analysis of selected mutants with eukaryotic cell biology and proteomics, we demonstrate that EccE(1) is critical for ESX-1 function, secretion of effector proteins, and pathogenesis. Our research improves knowledge of the molecular basis of M. tuberculosis virulence and enhances our understanding of pathogenesis.Microbiologyesx-1 secretion systemecce1mycobacterium tuberculosismembrane proteinsvirulencevii secretion systemesat-6expressiontranscriptomeattenuationresistancepathwaycompleximpacttext::journal::journal article::research article