De Biasi, FedericoMascitti, Beatrice BernadetteKupce, EriksRastrelli, Federico2022-05-092022-05-092022-05-092022-05-0110.1016/j.jmr.2022.107190https://infoscience.epfl.ch/handle/20.500.14299/187663WOS:000783302300003In the study of small molecule ligands and candidate macromolecular targets, water spins in long-lived association with macromolecules (proteins or nanoparticles) constitute a remarkable source of magneti-zation that can be exploited to reveal ligand-target binding. In this work we show how the selective sat-uration of water spins complemented with adiabatic off-resonance spin-locks can remove the NOE contribution of bulk water in the final difference spectrum, leading to uniformly enhanced signals that reveal weak ligand-target interactions.(c) 2022 Elsevier Inc. All rights reserved.Biochemical Research MethodsPhysics, Atomic, Molecular & ChemicalSpectroscopyBiochemistry & Molecular BiologyPhysicsnuclear overhauser effectligand bindingadiabatic spin -locksaturation transferwater suppressionspin-echobindingtext::journal::journal article::research article