Villalba, Maria I.Rossetti, EugeniaBonvallat, AllanYvanoff, CharlotteRadonicic, VjeraWillaert, Ronnie G.Kasas, Sandor2023-08-142023-08-142023-08-142023-04-2510.1073/pnas.2221284120https://infoscience.epfl.ch/handle/20.500.14299/199789WOS:001038053200002Antibiotic resistance is nowadays a major public health issue. Rapid antimicrobial susceptibility tests (AST) are one of the options to fight this deadly threat. Performing AST with single-cell sensitivity that is rapid, cheap, and widely accessible, is chal-lenging. Recent studies demonstrated that monitoring bacterial nanomotion by using atomic force microscopy (AFM) upon exposure to antibiotics constitutes a rapid and highly efficient AST. Here, we present a nanomotion detection method based on optical microscopy for testing bacterial viability. This novel technique only requires a very basic microfluidic analysis chamber, and an optical microscope equipped with a camera or a mobile phone. No attachment of the microorganisms is needed, nor are specific bacterial stains or markers. This single-cell technique was successfully tested to obtain AST for motile, nonmotile, gram-positive, and gram-negative bacteria. The simplicity and efficiency of the method make it a game-changer in the field of rapid AST.Multidisciplinary SciencesScience & Technology - Other Topicsnanomotionbacteriaantibioticoptical microscopytext::journal::journal article::research article