Haruki, HirohitoGonzalez, Monica RengifoJohnsson, Kai2012-07-132012-07-132012-07-13201210.1371/journal.pone.0037598https://infoscience.epfl.ch/handle/20.500.14299/83821WOS:000305338500043We introduce three assays for analyzing ligand-receptor interactions based on the specific conjugation of ligands to SNAP-tag fusion proteins. Conjugation of ligands to different SNAP-tag fusions permits the validation of suspected interactions in cell extracts and fixed cells as well as the establishment of high-throughput assays. The different assays allow the analysis of strong and weak interactions. Conversion of ligands into SNAP-tag substrates thus provides access to a powerful toolbox for the analysis of their interactions with proteins.Throughput Screening AssaysDihydrofolate-ReductaseFluorescence PolarizationEscherichia-ColiSmall MoleculesIn-VivoBindingExpressionAffinityCellstext::journal::journal article::research article