Caen, OurielSchutz, SimonJammalamadaka, M. S. SuryatejaVrignon, JeremyNizard, PhilippeSchneider, Tobias M.Baret, Jean-ChristopheTaly, Valerie2018-12-132018-12-132018-12-132018-10-2210.1038/s41378-018-0033-2https://infoscience.epfl.ch/handle/20.500.14299/152346WOS:000447751200001Fluorescence-activated droplet sorting (FADS) is one of the most important features provided by droplet-based microfluidics. However, to date, it does not allow to compete with the high-throughput multiplexed sorting capabilities offered by flow cytometery. Here, we demonstrate the use of a dielectrophoretic-based FADS, allowing to sort up to five different droplet populations simultaneously. Our system provides means to select droplets of different phenotypes in a single experimental run to separate initially heterogeneous populations. Our experimental results are rationalized with the help of a numerical model of the actuation of droplets in electric fields providing guidelines for the prediction of sorting designs for upscaled or downscaled microsystems.Nanoscience & NanotechnologyInstruments & InstrumentationScience & Technology - Other Topicsprotein-signaling networksdirected enzyme evolutionsingle-cellmammalian-cellsflow-cytometrymicrofluidicssortermanipulationselectionbreakuptext::journal::journal article::research article