Ivanov, DenisShcheslavskiy, VladislavMärki, IwanLeutenegger, MarcelLasser, Theo2009-03-132009-03-132009-03-13200910.1117/12.807596https://infoscience.epfl.ch/handle/20.500.14299/35966WOS:000285712600009We present the results on two-photon total-internal-reflection fluorescence correlation spectroscopy. The combination of liquid crystal spatial light modulator, providing radial polarization, with ultrafast laser (picosecond Nd:GdVO4 laser) allowed us to take the advantage of nonlinear optical contrast mechanisms to suppress the side-lobe energy specific for radial polarization and reduce the effective excited volume twice compared to one-photon evanescent wave excitation in fluorescence correlation spectroscopy.Fluorescence correlation spectroscopytwo-photon microscopytotal internal reflectionultrafast laserradial polarisationMicroscopyExcitationHigh volume confinement in two-photon fluorescence correlation spectroscopy with radially polarized lighttext::conference output::conference proceedings::conference paper