Lukinavičius, GražvydasReymond, LucUmezawa, KeitaroSallin, OlivierD’Este, ElisaGöttfert, FabianTa, HaisenHell, Stefan W.Urano, YasuteruJohnsson, Kai2016-07-272016-07-272016-07-27201610.1021/jacs.6b04782https://infoscience.epfl.ch/handle/20.500.14299/128157WOS:000381062600008Here we present a far-red, silicon-rhodamine-based fluorophore (SiR700) for live-cell multicolor imaging. SiR700 has excitation and emission maxima at 690 nm and 715 nm, respectively. SiR700-based probes for F-actin, microtubules, lysosomes and SNAP-tag are fluorogenic, cell-permeable and compatible with superresolution microscopy. In conjunction with probes based on the previously introduced carboxy-SiR650, SiR700-based probes permit multicolor live-cell superresolution microscopy in the far-red, thus significantly expanding our capacity for imaging living cells.text::journal::journal article::research article