Liu, ChenMalek, MichaelPoon, IvanJiang, LanzhouSiddiquee, Arif M.Sheppard, Colin J. R.Roberts, AnnQuiney, HarryZhang, DouguoYuan, XiaocongLin, JiaoDepeursinge, ChristianMarquet, PierreKou, Shan Shan2019-09-192019-09-192019-09-192019-09-0110.1364/PRJ.7.001042https://infoscience.epfl.ch/handle/20.500.14299/161285WOS:000484142400014We report a dual-contrast method of simultaneously measuring and visualizing the volumetric structural information in live biological samples in three-dimensional (3D) space. By introducing a direct way of deriving the 3D scattering potential of the object from the synthesized angular spectra, we obtain the quantitative subcellular morphology in refractive indices (RIs) side-by-side with its fluorescence signals. The additional contrast in RI complements the fluorescent signal, providing additional information of the targeted zones. The simultaneous dual-contrast 3D mechanism unveiled interesting information inaccessible with previous methods, as we demonstrated in the human immune cell (T cell) experiment. Further validation has been demonstrated using a Monte Carlo model. (C) 2019 Chinese Laser Press.Opticselectron-microscopyphasereconstructionfieldtext::journal::journal article::research article