Leman, Julia KoehlerWeitzner, Brian D.Lewis, Steven M.Adolf-Bryfogle, JaredAlam, NawsadAlford, Rebecca F.Aprahamian, MelanieBaker, DavidBarlow, Kyle A.Barth, PatrickBasanta, BenjaminBender, Brian J.Blacklock, KristinBonet, JaumeBoyken, Scott E.Bradley, PhilBystroff, ChrisConway, PatrickCooper, SethCorreia, Bruno E.Coventry, BrianDas, RhijuDe Jong, Rene M.DiMaio, FrankDsilva, LornaDunbrack, RolandFord, Alexander S.Frenz, BrandonFu, Darwin Y.Geniesse, CalebGoldschmidt, LukaszGowthaman, RagulGray, Jeffrey J.Gront, DominikGuffy, SharonHorowitz, ScottHuang, Po-SsuHuber, ThomasJacobs, Tim M.Jeliazkov, Jeliazko R.Johnson, David K.Kappel, KalliKaranicolas, JohnKhakzad, HamedKhar, Karen R.Khare, Sagar D.Khatib, FirasKhramushin, AlisaKing, Indigo C.Kleffner, RobertKoepnick, BrianKortemme, TanjaKuenze, GeorgKuhlman, BrianKuroda, DaisukeLabonte, Jason W.Lai, Jason K.Lapidoth, GideonLeaver-Fay, AndrewLindert, SteffenLinsky, ThomasLondon, NirLubin, Joseph H.Lyskov, SergeyMaguire, JackMalmstroem, LarsMarcos, EnriqueMarcu, OrlyMarze, Nicholas A.Meiler, JensMoretti, RoccoMulligan, Vikram KhippleNerli, SantruptiNorn, ChristofferO'Conchuir, ShaneOllikainen, NoahOvchinnikov, SergeyPacella, Michael S.Pan, XingjiePark, HahnbeomPavlovicz, Ryan E.Pethe, ManasiPierce, Brian G.Pilla, Kala BharathRaveh, BarakRenfrew, P. DouglasBurman, Shourya S. RoyRubenstein, AlizaSauer, Marion F.Scheck, AndreasSchief, WilliamSchueler-Furman, OraSedan, YuvalSevy, Alexander M.Sgourakis, Nikolaos G.Shi, LeiSiegel, Justin B.Silva, Daniel-AdrianoSmith, ShannonSong, YifanStein, AmelieSzegedy, MariaTeets, Frank D.Thyme, Summer B.Wang, Ray Yu-RueiWatkins, AndrewZimmerman, LiorBonneau, Richard2020-06-142020-06-142020-06-142020-06-0110.1038/s41592-020-0848-2https://infoscience.epfl.ch/handle/20.500.14299/169284WOS:000537047600003The Rosetta software for macromolecular modeling, docking and design is extensively used in laboratories worldwide. During two decades of development by a community of laboratories at more than 60 institutions, Rosetta has been continuously refactored and extended. Its advantages are its performance and interoperability between broad modeling capabilities. Here we review tools developed in the last 5 years, including over 80 methods. We discuss improvements to the score function, user interfaces and usability. Rosetta is available at .This Perspective reviews tools developed over the past five years in the macromolecular modeling, docking and design software Rosetta.Biochemical Research MethodsBiochemistry & Molecular Biologyprotein-structure predictionde-novo designhoming endonuclease specificitysmall-molecule dockingsparse nmr datacomputational designatomic-accuracyantibody structuresfold determinationvariable regiontext::journal::journal article::research article