Shen, XiaoHacker, David L.Baldi, LuciaWurm, Florian M.2014-01-232014-01-232014-01-23201310.1186/1753-6561-7-S6-P19https://infoscience.epfl.ch/handle/20.500.14299/100125Insect cells have been widely used for the production of recombinant proteins using recombinant baculovirus for gene delivery [1]. To simplify protein production in insect cells, we have previously described a method, based on transient gene expression (TGE) with cultures of suspension-adapted Sf9 cells using polyethylenimine (PEI) for DNA delivery [2]. Expression of GFP has been realized at high efficiency and a tumor necrosis factor receptor-Fc fusion protein (TNFR-Fc) was produced at a level of 40 mg/L. However, the efficiency of the insect cells TGE system has not been studied and further opti- mization may improve protein titers. Here, we studied the efficiency of PEI for plasmid delivery in Sf9 cells.text::conference output::conference proceedings::conference paper