Descloux, AdrienMueller, MarcelNavikas, VytautasMarkwirth, AndreasVan den Eynde, RobinLukes, TomasHuebner, WolfgangLasser, TheoRadenovic, AleksandraDedecker, PeterHuser, Thomas R.2020-03-032020-03-032020-03-032020-01-0110.1515/nanoph-2019-0346https://infoscience.epfl.ch/handle/20.500.14299/166792WOS:000508009000011Super-resolution structured illumination microscopy (SR-SIM) can be conducted at video-rate acquisition speeds when combined with high-speed spatial light modulators and sCMOS cameras, rendering it particularly suitable for live-cell imaging. If, however, three-dimensional (3D) information is desired, the sequential acquisition of vertical image stacks employed by current setups significantly slows down the acquisition process. In this work, we present a multiplane approach to SR-SIM that overcomes this slowdown via the simultaneous acquisition of multiple object planes, employing a recently introduced multiplane image splitting prism combined with highspeed SIM illumination. This strategy requires only the introduction of a single optical element and the addition of a second camera to acquire a laterally highly resolved 3D image stack. We demonstrate the performance of multiplane SIM by applying this instrument to imaging the dynamics of mitochondria in living COS-7 cells.Nanoscience & NanotechnologyMaterials Science, MultidisciplinaryOpticsPhysics, AppliedScience & Technology - Other TopicsMaterials ScienceOpticsPhysicssuper-resolution optical microscopypacs1042.30.wbmultiplane image acquisitionstructured illumination microscopyfluorescence microscopytext::journal::journal article::research article