Mason, G. F.Gruetter, R.Rothman, D. L.Behar, K. L.Shulman, R. G.Novotny, E. J.2012-05-262012-05-262012-05-26199510.1038/jcbfm.1995.2https://infoscience.epfl.ch/handle/20.500.14299/8087213C isotopic tracer data previously obtained by 13C nuclear magnetic resonance in the human brain in vivo were analyzed using a mathematical model to determine metabolic rates in a region of the human neocortex. The tricarboxylic acid (TCA) cycle rate was 0.73 ± 0.19 μmol min-1 g-1 (mean ± SD; n = 4). The standard deviation reflects primarily intersubject variation, since individual uncertainties were low. The rate of α- ketoglutarate/glutamate exchange was 57 ± 26 μmol min-1 g-1 (n = 3), which is much greater than the TCA cycle rate; the high rate indicates that α-ketoglutarate and glutamate are in rapid exchange and can be treated as a single combined kinetic pool. The rate of synthesis of glutamine from glutamate was 0.47 μmol min-1 g-1 (n = 4), with 95% confidence limits of 0.139 and 3.094 μmol min-1 g-1; individual uncertainties were biased heavily toward high synthesis rates. From the TCA cycle rate the brain oxygen consumption was estimated to be 2.14 ± 0.48 μmol min-1 g-1 (5.07 ± 1.14 ml 100 g-1 min-1; n = 4), and the rate of brain glucose consumption was calculated to be 0.37 ± 0.08 μmol min-1 g-1 (n = 4). The sensitivity of the model to the assumptions made was evaluated, and the calculated values were found to be unchanged as long as the assumptions remained near reported physiological values.text::journal::journal article::research article