Bila, HaleKurisinkal, Eva E.Bastings, Maartje M. C.2019-06-182019-06-182019-06-182019-02-0110.1039/c8bm01249khttps://infoscience.epfl.ch/handle/20.500.14299/156847WOS:000457525700007DNA as a biomaterial has evoked great interest as a potential platform for therapeutics and diagnostics and as hydrogel scaffolds due to the relative ease of programming its robust and uniform shape, site-specific functionality and controlled responsive behavior. However, for a stable self-assembled product, a relatively high cation concentration is required to prevent denaturation. Physiological and cell-culture conditions do not match these concentrations and present additional nucleases that cause a serious threat to the integrity of DNA-based materials. For the translation of this promising technology towards bioengineering challenges, stability needs to be guaranteed. Over the past years, various methods have been developed addressing the stability-related weaknesses of DNA-origami. This mini-review explains the common stability issues and compares the stabilization strategies recently developed. We present a detailed overview of each method in order to ease the selection process on which method to use for future users of DNA-origami as a biomaterial.Materials Science, BiomaterialsMaterials Scienceantisense oligonucleotidesin-vivonanostructuresstabilitynanoparticlesdegradationexonucleasepolymernanomaterialsencapsulationtext::journal::journal article::review article