Vinchhi, BakulBoss, ChristopheHermant, AurelieBouche, Nicolasde Marchi, UmbertoDehollain, Catherine2020-07-162020-07-162020-07-162019-01-0110.1109/SENSORS43011.2019.8956793https://infoscience.epfl.ch/handle/20.500.14299/170142WOS:000534184600301An optical pancreatic beta-cell based biosensor for measuring cytosolic [Ca2+] is presented in this work. An in vitro bio-electronic system is built for testing various applications and characterizing features of the biosensor. A panoply of applications such as effect of temperature and response of the cells in culture media with 11 mM glucose vs. KRBH with 0 mM glucose are explored. The effect of the excitation light intensity and integration time on the fluorescence performance of the biosensor FRET probe are elucidated experimentally.Engineering, Electrical & ElectronicRemote SensingEngineeringfluorescenceoptical biosensorfretglucose stimulationglucose monitoringpancreatic beta-cellstemperaturegenetically inducedtext::conference output::conference proceedings::conference paper