The biosynthetic routes for carbon monoxide and cyanide in the Ni-Fe active site of hydrogenases are different

Roseboom, WinfriedBlokesch, MelanieBöck, AugustAlbracht, Simon P. J.2009-05-152009-05-152009-05-15200510.1016/j.febslet.2004.12.013https://infoscience.epfl.ch/handle/20.500.14299/40105The incorporation of carbon into the carbon monoxide and cyanide ligands of [NiFe]-hydrogenases has been investigated by using (13)C labelling in infrared studies of the Allochromatium vinosum enzyme and by (14)C labelling experiments with overproduced Hyp proteins from Escherichia coli. The results suggest that the biosynthetic routes of the carbon monoxide and cyanide ligands in [NiFe]-hydrogenases are different.The biosynthetic routes for carbon monoxide and cyanide in the Ni-Fe active site of hydrogenases are differenttext::journal::journal article::research article