Alpern, DanielGardeux, VincentRusseil, JulieMangeat, BastienMeireles-Filho, Antonio C. A.Breysse, RomaneHacker, DavidDeplancke, Bart2019-06-182019-06-182019-06-182019-04-1910.1186/s13059-019-1671-xhttps://infoscience.epfl.ch/handle/20.500.14299/157174WOS:000465144800001Despite its widespread use, RNA-seq is still too laborious and expensive to replace RT-qPCR as the default gene expression analysis method. We present a novel approach, BRB-seq, which uses early multiplexing to produce 3 cDNA libraries for dozens of samples, requiring just 2hours of hands-on time. BRB-seq has a comparable performance to the standard TruSeq approach while showing greater tolerance for lower RNA quality and being up to 25 times cheaper. We anticipate that BRB-seq will transform basic laboratory practice given its capacity to generate genome-wide transcriptomic data at a similar cost as profiling four genes using RT-qPCR.Biotechnology & Applied MicrobiologyGenetics & HeredityBiotechnology & Applied MicrobiologyGenetics & Heredityrna-seqtranscriptomicsgene expressionqpcrbarcodingtext::journal::journal article::research article