Adameyko, IgorBakken, TrygveBhaduri, AparnaChhatbar, ChintanFilbin, Mariella G.Gate, DavidHochgerner, HannahKim, Chang NamKrull, JordanLa Manno, GioeleLi, QingyunLinnarsson, StenMa, QinMayer, ChristianMenon, VilasNano, PatriciaPrinz, MarcoQuake, SteveWalsh, Christopher A.Yang, JinBayraktar, Omer AliGokce, OzgunHabib, NaomiKonopka, GenevieveLiddelow, Shane A.Nowakowski, Tomasz J.2024-12-242024-12-242024-12-192024-12-0310.1038/s41593-024-01827-9https://infoscience.epfl.ch/handle/20.500.14299/242472WOS:001369839700028Single-cell and single-nucleus genomic approaches can provide unbiased and multimodal insights. Here, we discuss what constitutes a molecular cell atlas and how to leverage single-cell omics data to generate hypotheses and gain insights into cell transitions in development and disease of the nervous system. We share points of reflection on what to consider during study design and implementation as well as limitations and pitfalls.EnglishSOMATIC MUTATIONMOSAIC MUTATIONSLINEAGE ANALYSISRNA-SEQMOUSEMICROGLIANEURONSCORTEXTAXONOMYATLASScience & TechnologyLife Sciences & BiomedicineApplying single-cell and single-nucleus genomics to studies of cellular heterogeneity and cell fate transitions in the nervous systemtext::revue::article de revue